案例

(
节选于我们修改过的稿件。蓝字是编辑加的。)
1.
To
determine whether the constructed plasmid
was efficientworks
well, we first transfected the plasmid with liposomes into
cultured SH-SY5Y cells for 24 hours
and
analyzed
the expression of corresponding
proteins byused
western blot analysis
using anwith
antibody against EGFP
to check the expression of corresponding proteins.
2.
We screened aA
pool of
Arabidospis
Arabidopsis
lines transformed with the activation vector
was screened
for short hypocotyl mutants under dim far-red light.
Consequently we
isolated,
and
three mutant lines designated
chibi1-3
(chi)
were
isolated.
3. Rats were
sacrificed
killed
at different time points
followingof
reperfusion.
Left hemispheric
Ffrontal
and parietal lobe cortex
tissues
on
the left hemisphere (6 to 11 mm
fromto
the tip of olfactory bulb) were separated and saved in liqguid
nitrogen.
4.
Rats
which
were supplemented
with further injections as necessary during experiments
to
sustain
a
level of
acceptable
anesthesia. Body temperature was maintained at 36-37¡ãC
using a heating pad
placed underneath the rat.
. Each rat’sThe
heads
was
wasere
fixed horizontally in a stereotaxic instrument (Narishige,
Japan). The skull was exposed,
and
a small burr hole was made
at the
appropriate
coordinates
drilled
to enable vertical penetration by
the stimulating and recording
electrodes. All electrodes were zeroed
on the
at bregma,
and all coordinates were calculated from
this point.
5.
We
These experiments demonstrated a distinctive localization of OMPFC
neurons, which revealed excitatory responses to BLA stimulation, although
Pérez-Jaranay
and Vivesa
previous study did not
demonstrate this apparent distribution of the excitatory responses (P¨¦rez-Jaranay
and Vives, 1991).
6.
WHere
we suggest
that a
comprehensive molecular genetic study involving a large number of NF2
is
encouraged to build up our Chinese gene mutation database not only
for the benefit
for
ofthe
clinical
practice, but also for genetic counseling
purposess.
7.
It is
high
reasonable to
believe
suggest that these NATs are critical for the life of humans,
mouse
mice and rats,
just
because they
are stillremain
in the transcriptome after tens of million years of evolution.
8.
A total of 570 NATs were selected,
tripled
ofthree
times the number
lehner
reported
earlier
in a previous study (8),
and
with most of them
are thebeing
reported for the first time
to be reported.
9.
It was noted that
the onset of SWS1 expression in our study was earlier
than
the
that
one
reported previously [26]
where the expression initiateds
between 55 and 60 hpf
and
with about half of
the embryos showing
expression at 60 hpf but
are stillremaining
in stage 1.
In theirThe
aforementioned study
differed from ours in that only fish that had achieved
certain developmental characteristics at certain time points were examined for
opsin expression to minimize the individual variation [26],
while we did not perform any
such selection. The time
difference
discrepancy between the two studies is
most likely
to be due to this difference
originate from
it..
10. By
constructing DB2GO, the entries in the
BioDW
member databases
of BioDW are linked
organically to the terms of Gene
Ontology Database.
organically. In the DB2GO table,
Iit
is quite easy to find entries
in the DB2GO table
from different databases
as they,
which
all are
all
annotated by the same GO term,
from different databases.
11.
We screened aA
pool of Arabidospis
Arabidopsis
lines transformed
with the activation vector
was screened
for short
hypocotyl mutants under dim far-red light.
Consequently we isolated,
and
three mutant lines designated
chibi1-3
(chi)
were isolated.
12.
[Author:
You should mention the 3 treatment groups here since this is really part of the
study design (like you did in the ‘Methods’ section but in less detail).]
|